Dr. Racha Chouaib - د. رشا شعيب

علم الوراثة الجزيئية - Molecular Genetics
الرتبة : معيد
الفرع الأول

Résumé de carrière

Academic Qualification
- Post-Doctoral Position (2018), American University of Beirut, Beirut, Lebanon
- Post-Doctoral Position (2017), Université Pierre et Marie Curie – Sorbonne Universités, Paris, France
- PhD in Molecular Genetics, Biology-Health (2016), University of Montpellier, France
- Master 2 in Genomic and Health (2012), Lebanese University, Beirut, Lebanon
- Master 1 in Molecular and Cellular Biology (2011), Lebanese University, Beirut, Lebanon
- License in Biology (2010), Lebanese University, Beirut, Lebanon
- Bachelor in Life Science (2007), New Lebanon School, Nabatieh, Lebanon
Research Positions
- Post-Doctoral Position, Title: “The anti-cancer effect of Thymoquinone nanoparticles” , AUB (American University of Beirut), Beirut.
- Post-Doctoral Position, Title: “Analysis of the functions of ribonucleoprotein granules”, IBPS (Institut de Biologie Paris-Seine), at UPMC (Université Pierre et Marie Curie – Sorbonne Universités), Paris, France.
- Doctoral Position
Title: “Systematic analysis of mRNA localization in human cells by new approaches”, IGMM (Institut de Génétique Moléculaire de Montpellier), Montpellier, France.
- Master 2 traineeship, Pharmacogenetic research activities
Title: “Analysis of the power of induction of Cyclophosphamide on the CYP1A1 mRNA in the MCF7 cell line”, PRASE (Plateforme de Recherche et d’Analyse en Sciences de l’Environnement), Beirut, Lebanon.
Teaching positions
- Assistant Professor at the Lebanese University:
Instructor of “Molecular Pathology” and “Principles of Molecular Diagnostic”, Master II, Molecular Diagnostic and Forensic Sciences, Beirut.
- Assistant Professor at the Lebanese International Universities (LIU):
Instructor of Genetic (BIOL365), Developmental Biology (BIOL435), Molecular and Cellular Biology (BIOL275), General Biology (BIOL200), and Seminar (BIOL490), at Beirut, Saida, Nabatieh and Tyre Campuses.
Technical skills
- Molecular Biology: DNA extraction, RNA extraction, DNA microarrays, Recombinant DNA, PCR, qRT-PCR, Cloning, Crisper genome editing, Luciferase assay.
- Cellular Biology: Cell culture, Cell tracking, Isolation of clones, Wound healing assay, Single cell sorting, Cell cycle arrest.
- Transfection: Transient and stable transfection, Transfection of plasmids/siRNA, Electroporation.
- Imaging: Fluorescent and confocal Microscopy, Imagery in real time, Super-resolution microscopy, Fluorescent in situ Hybridization (FISH et smiFISH), Immunofluorescent assay.
- Nanoparticles preparation: FNP, DLS, HPLC.
- Software: Microsoft office, Adobe Photoshop/Illustrator, Fiji, Metamorph, Oligostan, IMGT ontology, FISH-Quant, GraphPad Prism, Icy.

Axes de recherche

• Chouaib R, Safieddine A, Pichon X, Kwon OS, Samacoits A, Traboulsi AM, Tsanov N, Robert MC, Poser I, Zimmer C, Hyman A, Le Hir H, Walter T, Zibara K, Mueller F, Peter M, Bertrand E. A localization screen reveals translation factories and widespread co-translational protein targeting. Submitted to Cell. (IF: 31.398)
• Garcia-Jove Navarro M, Kashida S, Chouaib R, Jover AB, Ipendey E, Saito H, Wang DO, Weil D and Gueroui Z. ArtiGranules, a mimicking tool to probe RNP granule assembly and regulation within the cell. Under review in Nature Communication. (IF: 12.124)
• Samacoits A, Chouaib R, Safieddine A, Traboulsi AM, Ouyang W, Zimmer C, Peter M, Bertrand E, Walter T, Mueller F. FISH-loc: a simulation and analysis framework to study sub-cellular mRNA localization. Nature Communication; 2018 Nov 2;9(1):4584. (IF: 12.124)
• Courel M, Bénard M, Ernoult-Lange M, Chouaib R, Hubstenberger A, Kress M, Weil D. PBodies: microscopic droplets that coordinate the cytoplasmic storage of mRNAs encoding regulatory proteins. Medicine Science (Paris). 2018 Apr;34(4):306-308. (IF: 2.3801)
• Hubstenberger A, Courel M, Bénard M, Souquère S, Ernoult-Lange M, Chouaib R, Yi Z, Morlot JB, Munier A, Fradet M, Daunesse M, Bertrand E, Pierron G, Mozziconacci J, Kress M, Weil D. P-body purification reveals the condensation of repressed mRNA regulons. Molecular Cell. 2017 Oct 5; 68(1): 144-157. e5. (IF: 14.714)
• Chouaib R*, Tsanov N*, Samacoits A*, Traboulsi AM, Gostan T, Weber C, Zimmer C, Zibara K, Walter T, Peter M, Bertrand E and Muller F. smiFISH and FISH-quant – a flexible single RNA detection approach with super-resolution capability. Nucleic Acids Research. 2016 Dec 15; 44(22): e165. (IF: 10.162)
• Pichon X, Bastide A, Safieddine A, Chouaib R, Samacoits A, Basyuk E, Peter M, Mueller F and Bertrand E. Visualization of single endogenous polysomes reveals the dymanics of translation in live human cells. Journal of Cell Biology. 2016 Sep 12; 214(6):769-81. (IF: 7.955)
• Tantale K, Mueller F, Kozulic-Pirher A, Lesne A, Victor JM, Robert MC, Capozi S, Chouaib R, Bäcker V, Mateos-Langerak J, Darzacq X, Zimmer C, Basyuk E, Bertrand E. A single-molecule view of transcription reveals convoys of RNA polymerases and multi-scale bursting. Nature Communication. 2016 Jul 27; 7:12248. (IF: 12.124)
• The Twentieth Annual Meeting of the RNA Society (RNA meeting, 2015): “Subcellular localization of human motor mRNAs at the level of single molecules”, University of Wisconsin, Madison, USA.
• Tiny European RNA Meeting (TERM13, 2014): “Toward a systematic analysis of mRNA localization in human cells”, Jacques Monod Institute, Paris, France.
• CBS2 annual Meeting (2013): “mRNA Analysis at the level of single molecules”, University of Montpellier, Montpellier, France
Superior qualification and joint doctoral scholarship for three years from the Lebanese University, University of Montpellier and the Lebanese National Council for Scientific Research (LNCSR).